Fig. 4
Perioperative SD increases the activity of HVA calcium channels and L-type calcium channels and excitability in large DRG neurons at 9 days after surgery. a Representative trace of HVA calcium channels in large DRG neurons before and after bath perfusion of 1 μm nifedipine. b Quantitation of the results in A (5 to 6 rats per group; 13 neurons from sham group, 15 neurons from sham+SD group, 15 neurons from incision group, 19 neurons from incision+SD group). One-way ANOVA followed by a post hoc Tukey test: Fbaseline (3, 58) = 2.903, Fnifedipine (3, 58) = 0.461, *p < 0.05 for the incision group vs. the incision+SD group. c Effect of nifedipine on channel activity. Numbers of neurons and rats were the same as in B. One-way ANOVA followed by a post hoc Tukey test: F (3, 58) = 3.223, *p < 0.05 for the incision group vs. the incision+SD group. d Resting membrane potentials (left) and current threshold needed to evoke the first action potential (right) from large DRG neurons (5 to 6 rats per group, sham group: n = 21; sham+SD: n = 16; incision group: n = 18; incision+SD group: n = 21). One-way ANOVA followed by a post hoc Tukey test: FRMP (3, 72) = 10.95; FCT (3, 72) = 10.44. **p < 0.01 for the incision group vs. the incision+SD group. e Representative traces of evoked action potentials from DRG neurons. f Effect of current intensity on the number of evoked action potentials in large DRG neurons. Numbers of rats and neurons were the same as in D. Two-way ANOVA followed by a post hoc Tukey test: Fgroup (3, 1008) = 96.37, *p < 0.05, **p < 0.01 for the incision group vs. the incision+SD group at each stimulation intensity