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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Sleep deprivation of rats increases postsurgical expression and activity of L-type calcium channel in the dorsal root ganglion and slows recovery from postsurgical pain

Fig. 3

Perioperative SD increases expression of L-type HVA channels in lumbar (L4–6) DRG neurons, mainly in medium/large cells. a Representative immunofluorescence results, showing higher expression of L-type channels on the neuronal membranes of rats in the incision+SD group than the incision group. Scale bar: 100 μm. b Quantitation of the results in A (10 rats per group). One-way ANOVA followed by a post hoc Tukey test: F(3, 8) = 74.13, **p < 0.01 for the incision group vs. the incision+SD group. c Size distribution of L-type-channel (Cav1.2)-labelled neuronal somata in the DRG (small: 6.59%, medium: 42.86%, large: 50.55%). d Representative immunofluorescence results showing strong co-expression of the L-type channel (Cav1.2) with neurofilament 200 (top row), but little or no co-expression with calcitonin gene-related peptide (CGRP; middle row), or isolectin B4 (IB4; bottom row). Arrows indicate co-labeling. Co-labeling analysis used the colocalization finder in Image J. Pearson’s correlation coefficients (r) are indicated. Scale bar: 50 μm

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