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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Polarizing receptor activation dissociates fibroblast growth factor 2 mediated inhibition of myelination from its neuroprotective potential

Fig. 2

N-terminal truncation of FGF2 abrogates its ability to inhibit myelination without disrupting its mitogenic activity. a Myelinating rat CNS cultures were treated with 100 ng/ml FGF2 or F2 V2 for 10 days (DIV18–28); provided are representative images demonstrating the differential effects of FGF2 and F2 V2 on myelination rates determined by MOG pos. sheets. Bars represent 100 μm. b In cultures treated with FGF2 or F2 V2 for 10 days FGF2, but not F2 V2 significantly inhibits myelination as determined by percentage of MOG pos. sheets/ SMI31 (one-way ANOVA). c Both FGF2 and F2 V2 significantly increase the number of Olig2+ and O4+ cells (one-way ANOVA). d Blocking antibodies directed towards FGFR3 (PRO-001) or towards FGFR2/3 (PRO-007) have different effects on the inhibition of myelination by FGF2, with blocking FGFR2/3 abrogating the effect of FGF2 (one-way ANOVA, p < 0.01 for FGF2 vs FGF2 + αFGFR2/3). e, f Proliferation of immunopurified A2B5+ OPCs in the presence or absence of FGF2 or F2 V2 (100 ng/ml). e Both FGF2 and F2 V2 act as strong mitogens on purified OPCs cultured for 6 days in differentiation media (modified Sato’s medium) as cell numbers (DAPI staining) significantly (one-way ANOVA) increase 3.4 or 2.7 times after treatment with FGF2 or F2 V2 respectively. f A2B5+ progenitors were maintained in modified Sato’s medium in the presence of FGF2 or F2 V2 for 72 h and proliferation was determined by EdU incorporation. There is no significant difference in the ability of FGF2 and F2 V2 to maintain OPC proliferation (one-way ANOVA). g A2B5+ progenitors were cultured for 6 days in modified Sato’s medium and the amount of O4+, PLP+ or MOG+ pos. cells (% of DAPI) was quantified. FGF2 treatment significantly reduced O4 expression (p < 0.05, one-way ANOVA) and almost abolished PLP and MOG expression (p < 0.001 for both markers, one-way ANOVA). Data are presented as means ± SEM from at least 3 independent experiments; * p < 0.05; ** p < 0.01; *** p < 0.001; ns – not significant

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