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Fig. 8 | Acta Neuropathologica Communications

Fig. 8

From: Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene

Fig. 8

Oxygen consumption and extracellular acidification rates of control and POLG1 p.Q811R variant fibroblasts and iPSC-derived astrocytes. a Oxygen consumption rates (OCRs) and extracellular acidification rates (ECARs) of 100 DIV POLG1 variant and healthy control astrocytes, normalized to protein content. OCR was used to calculate basal respiration, ATP-coupled and uncoupled respiration and spare respiratory capacity; glycolysis and glycolytic reserve were calculated from ECAR. Results are presented as ± SEM, n = 3 independent experiments; * indicates P < 0.05; ** indicates P < 0.01; unpaired t-test. b Oxygen consumption rates (OCRs) and extracellular acidification rates (ECARs) of cultured fibroblasts from healthy control and POLGQ811R patients, normalized to protein content. OCR was used to calculate basal respiration, ATP-coupled and uncoupled respiration and spare respiratory capacity; glycolysis and glycolytic reserve were calculated from ECAR. Results are presented as ± SEM, n = 3 independent experiments; unpaired t-test

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