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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Cellular alterations identified in pluripotent stem cell-derived midbrain spheroids generated from a female patient with progressive external ophthalmoplegia and parkinsonism who carries a novel variation (p.Q811R) in the POLG1 gene

Fig. 3

Western blot analysis reveals presence of oligomeric species of alpha-synuclein of high molecular weight in POLGQ811R variant MDNS. a Immunostaining of pan-α-synuclein in POLG1 variant and healthy control MDNS dissociated cells 106 DIV. Scale bar: 100 μm. b Quantification of aSYN-positive cells relative to total number of DAPI-labeled cells, in POLG1 variant and healthy control cultures. Results are presented as ± SEM; n = 4 independent experiments; unpaired t-test. c Expression of monomeric alpha-synuclein, phosphorylated (pS129) alpha-synuclein, and oligomeric (ASyO5) alpha-synuclein in POLG1 variant and healthy control MDNS. d Protein expression levels from (b) normalized to b-ACTIN. Results are presented as ± SEM; n = 4 independent experiments. ** indicates p < 0.01; unpaired t-test

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Acta Neuropathologica Communications

ISSN: 2051-5960

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