Table 2 Information of primary antibodies used for the study
Antigen | Antibody | Supplier | Cat#. | Species/Type | Application | Dilution |
|---|---|---|---|---|---|---|
Progranulin | PGRN | R&D | AF2420 | Goat/Polyclonal | IHC | 1:4000 |
Systems | FIHC | 1:1500 | ||||
WB | 1:2000 | |||||
IP | 2 μg | |||||
Amyloid beta | 6E10 | Biolegend | 803001 | Mouse/Monoclonal | IHC | 1:2000 |
(1–16) | FIHC | 1:1000 | ||||
WB | 1:1000 | |||||
CD45 | CD45/HI30 | Biolegend | 304001 | Mouse/Monoclonal | IHC | 1:2000 |
FIHC | 1:1000 | |||||
PHF-Tau (Ser202/Thr205) | AT8 | ThermoFisher | MN1020 | Mouse/Monoclonal | IHC | 1:2000 |
PHF-Tau | AT180 | Thermo | MN1040 | Mouse/Monoclonal | FIHC | 1:2000 |
(Thr231) | Fisher | WB | 1:2000 | |||
IBA1 | IBA1 | Fujifilm | 019–19,741 | Rabbit/Polyclonal | FIHC | 1:1000 |
CD31 | CD31 JC/70A | Abcam | Ab9498 | Mouse/Monoclonal | FIHC | 1:500 |
GFAP | GFAP | BD bioscience | 556330 | Mouse/Monoclonal | FIHC | 1:1000 |
LAMP1 | LAMP1 | Sigma | L1418 | Rabbit/Polyclonal | FIHC | 1:1000 |
CD68 | CD68 | Biolegend | 916104 | Mouse/Monoclonal | FIHC | 1:1000 |
Prosaposin | PSAP | R&D | AF8520 | Rabbit/Polyclonal | FIHC | 1:2000 |
Systems | WB | 1:10000 | ||||
IP | 2 μg | |||||
Sortilin [53] | Sortilin NT3 | Abcam | Ab16640 | Rabbit/Polyclonal | FIHC | 1:1000 |
WB | 1:2000 | |||||
TMEM106B [34] | TMEM106B | Bethyl Lab | A303- | Rabbit/Polyclonal | FIHC | 1:1000 |
439A-1 | WB | 1:1000 | ||||
Cathepsin D | cathepsin D | Cell signaling | #2284 | Rabbit/Polyclonal | WB | 1:1000 |
BACE1 | BACE1 | R&D | MAB931 | Mouse/Monoclonal | FIHC | 1:1000 |
Systems | WB | 1:2000 | ||||
Pan NF | SMI312 | Biolegend | 837904 | Mouse/Monoclonal | FIHC | 1:1000 |
Synaptophysin | SVP-38 | Sigma | S5768 | Mouse/Monoclonal | FIHC | 1:500 |
goat IgG | goat IgG | R&D | AB-108-C | Goat/Polyclonal | WB | 1:2000 |
Systems | IP | 2 μg |