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Table 2 Information of primary antibodies used for the study

From: Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer’s disease and aged brains: increased levels correlate with neuropathology

AntigenAntibodySupplierCat#.Species/TypeApplicationDilution
ProgranulinPGRNR&DAF2420Goat/PolyclonalIHC1:4000
  Systems  FIHC1:1500
     WB1:2000
     IP2 μg
Amyloid beta6E10Biolegend803001Mouse/MonoclonalIHC1:2000
(1–16)    FIHC1:1000
     WB1:1000
CD45CD45/HI30Biolegend304001Mouse/MonoclonalIHC1:2000
     FIHC1:1000
PHF-Tau (Ser202/Thr205)AT8ThermoFisherMN1020Mouse/MonoclonalIHC1:2000
PHF-TauAT180ThermoMN1040Mouse/MonoclonalFIHC1:2000
(Thr231) Fisher  WB1:2000
IBA1IBA1Fujifilm019–19,741Rabbit/PolyclonalFIHC1:1000
CD31CD31 JC/70AAbcamAb9498Mouse/MonoclonalFIHC1:500
GFAPGFAPBD bioscience556330Mouse/MonoclonalFIHC1:1000
LAMP1LAMP1SigmaL1418Rabbit/PolyclonalFIHC1:1000
CD68CD68Biolegend916104Mouse/MonoclonalFIHC1:1000
ProsaposinPSAPR&DAF8520Rabbit/PolyclonalFIHC1:2000
  Systems  WB1:10000
     IP2 μg
Sortilin [53]Sortilin NT3AbcamAb16640Rabbit/PolyclonalFIHC1:1000
     WB1:2000
TMEM106B [34]TMEM106BBethyl LabA303-Rabbit/PolyclonalFIHC1:1000
   439A-1 WB1:1000
Cathepsin Dcathepsin DCell signaling#2284Rabbit/PolyclonalWB1:1000
BACE1BACE1R&DMAB931Mouse/MonoclonalFIHC1:1000
  Systems  WB1:2000
Pan NFSMI312Biolegend837904Mouse/MonoclonalFIHC1:1000
SynaptophysinSVP-38SigmaS5768Mouse/MonoclonalFIHC1:500
goat IgGgoat IgGR&DAB-108-CGoat/PolyclonalWB1:2000
  Systems  IP2 μg