Fig. 5

Association of progranulin with lysosomal proteins. i) Progranulin and Lysosomal-Associated Membrane Protein-1 (LAMP-1) interactions in neurons and plaques. (a-c). PGRN (green) (A) and LAMP-1 (red) (b) immunoreactivity in neurons show almost complete colocalization (yellow), (c) showing PGRN in neurons is mainly localized to lysosomes. Scale bar represents 10 μm. (d-f). PGRN immunoreactivity (green) (d) and LAMP-1 (red) (E) in plaques of low plaque case showed limited colocalization (arrows-yellow) (F). Scale bar represents 30 μm. (g-i). PGRN immunoreactivity in plaques (green) (G) and LAMP-1 (red) (H) in plaques of high plaque case showed more extensive colocalization (arrows-yellow) (I). Scale bar represents 30 μm. (j-l). Limited immunoreactivity for LAMP-1 in AD cases (k). PGRN immunoreactivity in plaques (green) (j) and LAMP-1 (red) (k) in plaques of AD case showed very limited colocalization (arrows-yellow) (l). Scale bar represents 30 μm. II) Progranulin and lysosomal protein CD68 interactions in microglia and plaques. a-c). PGRN (green) (a) and CD68 (red) (b) immunoreactivities in microglia show almost complete colocalization (yellow) (c) showing PGRN in microglia is mainly localized to lysosomes. Plaque-associated CD68 and PGRN show almost complete colocalization Scale bar represents 10 μm. (d-f). Low plaque case: (g-i). High plaque case: (j-l). Alzheimer’s disease case. CD68 (red) and PGRN immunoreactivity (green) showed extensive colocalization (arrows-yellow) in all groups but most plaque-associated PGRN did not colocalize (green). Scale bar represents 30 μm.