Fig. 6

Inclusions are Thioflavin S positive and predominantly localized within neurons. a NeuN-positive cells (red), Thioflavin S (green) and DAPI-positive nuclei (blue) in the ipsilateral AON (monomers and all strains except ribbons) or OB (ribbons) at 3 MPI of the different strains or of the monomers. Photomicrographs were obtained from confocal stacks. The immunostaining was accomplished in two independent experiments, each on a half series of sections from 3 to 4 animals per group (3 MPI: monomers, n = 3; Fibrils, n = 4; Ribbons, n = 4; F-65, n = 4; F-91, n = 4; F-110, n = 4). Scale bars: 5 μm. b-d Staining of NeuN (green), Pser129 (red), Olig2 (white) and DAPI (blue) in the ipsilateral AON, at 6 MPI of the different strains. Orthogonal projections of Pser129+/NeuN+ cells (b) or olig2+ cells (c) and maximal projection images of pser129 processes-shaped inclusions (d) are obtained from confocal stacks. The immunodetection was performed in four independent experiments in one series of sections per animal, in 3–5 animals per group (6 MPI: monomers, n = 3; Fibrils, n = 5; Ribbons, n = 5; F-65, n = 4; F-91, n = 5; F-110, n = 5). Scale bar: 5 μm