Fig. 5
The density of α-syn inclusions in connected brain regions depends on the strain used. We measured the densitometry of pser129 staining in ipsilateral and contralateral AON (a), PC (b), Ent (c) at 3- and 6-MPI. Photomicrographs shown are representative of the average densitometry measured for each condition. Immunostaining for pser129 was performed in eight independent histochemical experiments. Densitometry performed on 3–5 animals per group (3 MPI: monomers, n = 4; Fibrils, n = 5; Ribbons, n = 5; F-65, n = 5; F-91, n = 5; F-110, n = 5; 6 MPI: monomers, n = 3; Fibrils, n = 5; Ribbons, n = 5; F-65, n = 4; F-91, n = 5; F-110, n = 5). Statistical analyses were performed by linear mixed-effect model and include three factors (strains, ipsilateral /contralateral sides, and delay post injection). Data are presented after log2-transformation. Boxes represent the 25th to 75th percentiles. The median and the mean are represented in each box by the line and the cross respectively. Error bars correspond to the minimal and maximal values measured. * comparison monomers to strains within same group of age and side; # comparison between strains within same group of age and side; + comparison between 3- and 6- MPI, same injectate, same side. Statistics are available in Additional file 5. Scale bar: 25 μm