Fig. 2

Strain-induced synucleinopathy propagates to interconnected brain regions with different spatial pattern depending on the strain. α-Syn inclusions detected in some ipsilateral (legend in blue) and contralateral (legend in red) brain regions at 3- and 6-MPI. α-Syn inclusions were detected by an antibody directed against α-syn phosphorylated on serine 129 (pser129). Immunostaining for pser129 was performed in eight independent histochemical experiments (3 MPI: monomers, n = 4; Fibrils, n = 5; Ribbons, n = 5; F-65, n = 5; F-91, n = 5; F-110, n = 5; 6 MPI: monomers, n = 3; Fibrils, n = 5; Ribbons, n = 5; F-65, n = 4; F-91, n = 5; F-110, n = 5). A list of brain structure abbreviations is provided as Additional file 4. Scale bar: 25 μm