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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Methylene blue inhibits Caspase-6 activity, and reverses Caspase-6-induced cognitive impairment and neuroinflammation in aged mice

Fig. 5

MB decreases microglial activation in aged Casp6 mice brains. Representative micrographs, Bar = 50 μm (a, d, g, j), quantitation of numbers of Iba1-immunopositive microglia numbers/mm2 (b, e, h, k) and subtype I-IV microglia (c, f, i, l) in hippocampus CA1 (a-c), corpus callosum (d-f), fimbria (g-i), and fornix (j-l) of vehicle-treated ACL/G (KI/Cre n = 5, KI/WT n = 3), WT/Cre (n = 3), WT/WT (n = 3) or MB-treated ACL/G (KI/Cre n = 2, KI/WT n = 1), WT/Cre (n = 3), and WT/WT (n = 3) mice. Data represents mean and s.e.m. Each symbol represents data from one mouse. Statistical analyses were conducted with one-way ANOVA or Brown-Forsythe ANOVA when variances were unequal, for e,h,k p < 0.0002, for Type I in (c,i,l) p < 0.02, for Type II in f,I,l p < 0.004, for Type IV in c,f,i p < 0.0.005; all others are NS. Post-hoc analyses were done with Dunnett’s test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs vehicle-treated WT/WT or vehicle-treated ACL/G # p < 0.05, # # p < 0.01, # # # p < 0.001

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