Fig. 5
From: A metastable subproteome underlies inclusion formation in muscle proteinopathies
Protein supersaturation is associated with downregulation. In this analysis, only proteins that are detected in HC, DC, AF, and RV, and for which there are defined σf scores in HC are included. a Folded supersaturation scores (σf) for the proteome (Prt) (N = 830) and proteins downregulated from HC to AF (N = 50). Box plots and statistical tests as in Fig. 1. b Percentage of proteins downregulated in the proteome (Prt) (48/830), proteins enriched in rimmed vacuoles (RV) (0/47), and top 5% most supersaturated proteins (based on HC context) (Top σf) (7/41). Significance determined by the Fisher Exact test, with Holm-Bonferroni correction. c Protein abundances in HC, DC, AF, and RV are plotted for the 47 proteins enriched in RVs included in the subset analyzed in this figure. Desmin is highlighted in yellow, the only RV-enriched protein whose abundance is increased between HC and AF. d Protein abundances in HC, DC, AF, and RV are plotted for the 47 proteins with the highest supersaturation scores (top 5%). Desmin again is highlighted in yellow, the only highly supersaturated protein with rising abundance. Those proteins that are significantly downregulated between HC and AF are highlighted in blue. In (c) and (d), the background black line and grey bar represent median and 25th–75th percentile range for the 830 proteins in the proteome in this subset. *p < 0.05, ****p < 0.0001