Acta Neuropathologica Communications

Table 3 Primer sequences used for qPCR

From: Regulation of microglial TMEM119 and P2RY12 immunoreactivity in multiple sclerosis white and grey matter lesions is dependent on their inflammatory environment

Primer	Primer sequence forward (5′- 3′)	Primer sequence reverse (3′-5′)
TMEM119	TCCAGGGTCAGATTACAAGAGCAC	ACTGTTGATTCTGGAGGGTTTGA
P2RY12	ACTCTCTCTTCCAGCCCAGGT	CCAGGACCAGTTCCTTGGCGTA
AIF-1	CCCTCCAAACTGGAAGGCTTCA	CTTTAGCTCTAGGTGAGTCTTGG
GFAP	GCAGATTCGAGAAACCAGCC	GCTCCTGCTTGGACTCCTTA
IL-1β	TACAGCTGGAGAGTGTAGATC	CAAATTCCAGCTTGTTATTG
MRC	AGTGATGGGACCCCTGTAACG	CCCAGTACCCATCCTTGCCTTT
SDHA^a	CCAGGGAAGACTACAAGGTGCGGA	AGGGTGTGCTTCCTCCAGTGCT
POLR2F^a	GAACTCAAGGCCCGAAAG	TGATGATGAGCTCGTCCAC

^a selected as housekeeping gene

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ISSN: 2051-5960

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