Table 2 Primary antibodies used for immunohistochemistry
Primary antibody | Ab Dilution | Antigen Retrieval | Source (article number) |
|---|---|---|---|
Rabbit anti TMEM119 C-terminus | 1:500 | Tris/EDTA pH 9 | Atlas Antibodies, Sweden (HPA051870) |
Rabbit anti Human P2Y12R C-terminus | 1:200 | Tris/EDTA pH 9 | Anaspec, Netherlands (AS-55042A) |
Mouse anti MHC-II (HLA-DR) | 1:1000 | Tris/EDTA pH 9 | Clone LN3, Pierce, ThermoFisher (MA5–11966) |
Rabbit anti Iba-1 | 1:1000 | Citrate pH 6 | WAKO Chemicals U.S.A. (019–19,741) |
Mouse anti PLP | 1:250 | Tris/EDTA pH 9 | Serotec (MCA839G) |
Rabbit anti CD3 | 1:100 | Citrate pH 6 | DAKO, Denmark (A04520) |
Mouse anti CD20 | 1:200 | Tris/EDTA pH 9 | DAKO, Denmark (M0755) |
Mouse anti IL-4 | 1:500 | Citrate pH 6 | BioMatik (CAU29167) |
Mouse anti IFN-γ | 1:000 | Tris/EDTA pH 9 | Abcam, U.K. (ab218426) |
Goat anti Iba-1 | 1:500 | Tris/EDTA pH 9 | Abcam, U.K. (ab5076) |