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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: The transcriptional coactivator and histone acetyltransferase CBP regulates neural precursor cell development and migration

Fig. 4

Neocortical layer V pyramidal cells show reduced volumes and alterations in spine density & morphology when missing CBP. a14 Neural differentiation in layer V of the neocortex, made visible through NeuN staining. Arrows mark lamina V giant pyramidal cells that appeared to be smaller and dysmorphic in transgenic mice. b1 3D cell reconstruction of Lamina V giant pyramidal cells from Hoechst and neurotracer stained 100 μm sections (b2) Determined mean cell volume in the lamina V of the neocortex at P30, significantly smaller in the transgenic mice compared to control litter mates. c14 Golgi-Cox impregnated pyramidal neurons in layer V show severe abnormalities and maturation deficits in hGFAP-cre::CBPFl/Fl mice. c5,6 The apical dendrite is significantly shorter and less dendritic spines can be observed. c7 The spine type distribution is shifted towards less mature spines. d13 Analysis in electron microscopic images of layer V neurons show that the number of synapses was significantly diminished in the cerebral cortex of hGFAP-cre::CBPFl/Fl mice. Scale bar: 50 μm (a1–4), 100 μm (c1–2), 10 μm (c3–4), 5 μm (d1–2); *p < 0.05, **p < 0.01

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