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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: The transcriptional coactivator and histone acetyltransferase CBP regulates neural precursor cell development and migration

Fig. 2

A tissue specific, early loss of CBP leads to structural alterations in the developing forebrain with impaired postnatal neurogenesis in the hippocampus and OB. a1,3 Sagittal H&E stained sections through the RMS at P30. Boxes mark the areas that were investigated subsequently. Arrows indicate representative size measurements of the neocortex thickness and OB width and length. Square brackets mark the CC thickness. b, f Mean neocortical thickness, CC thickness, dentate gyrus thickness as well as OB width and length are significantly smaller in hGFAP-cre::CBPFl/Fl animals aged 30 days. a2, a4 CBP stained regions of the hippocampus and neocortex displaying the reduction of CBP expression in the mouse model. a510 DCX staining, Ki67 staining and Sox2 staining of frontal sections through the dentate gyrus. c, d, e DCX+, Ki67+ and Sox2+ rates were significantly reduced in the SGZ of the dentate gyrus of hGFAP-cre::CBPFl/Fl mice at P30. a11-a16 Ki67, Sox2 and NeuN stained frontal sections through the left lateral ventricle and V-SVZ and the cell accumulation in mutant animals. In Ki67 stained sections V-SVZ typical areas are marked by arrows. Sox2 and NeuN stained frontal sections show different cytoarchitectural areas within the accumulation. a1722 NeuN neuronal staining, Calbindin staining of GABAergic periglomerular neurons and Tbr2 staining of glutamatergic juxtaglomerular neurons in the glomerular layer (GL) at P30. g Significantly lower rate of NeuN positive cells after early loss of CBP in the GL. h, i Whereas the rate of Calbindin positive inhibitory neurons is shown to be significantly reduced in the GL of the OB in hGFAP-cre::CBPFl/Fl mice, the rate of Tbr2 positive excitatory neurons is significantly increased compared to control animals. Scale bar: 2000 μm (a 1, 3), 200 μm (a 2, 4), 50 μm (a 5–10, 17–22), 150 μm (a 11–16); *p < 0.05, **p < 0.01, ***p < 0.001

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