Table 2 Antibodies used for Western blots
Protein | Manufacturer | Catalogue | Validation for WB (from manufacturers website) | RRID |
|---|---|---|---|---|
Actin | Abcam | AB 8226 | Cell lysates from HeLa, Jurkat, A431, HEK293, NIH 3 T3, PC12 cells. | AB_306371 |
Alpha synuclein | Santa Cruz | Sc-7011-R | SH-SY5Y cell lysate, α-synuclein expression in mouse and rat brain tissue extracts. | AB_2192953 |
Annexin V | Abcam | AB 54775 | ANXA5 expression in Hela, 293 T cell line, pentoxifylline cell treatment. | AB_940268 |
Beta Tubulin | Abcam | AB 18207 | HAP1 cell lysate; mouse brain and hippocampus lysates; rat brain lysate; human brain lysate. Knock out cell lines studies. | AB_444319 |
GAPDH | Abcam | AB 9845 | HeLa, A431, A549, NIH3T3, PC12 whole cell lysate | AB_307275 |
GFAP | Dako/Agilent | Z033429–2 | antibody solid-phase absorbed with human and cow serum proteins. One distinct precipitate (GFAP) with cow brain extract. | AB_10013382 |
Histone H3 | Abcam | Ab 1791 | HeLa, Drosophila embryo nuclear extract, NIH/3 T3, S.cerevisiae (Y190) and S.pombe whole cell | AB_302613 |
NMDANR2B | BD Biosciences | 610,416 | Rat NMDA receptors | AB_397796 |
PSD95 | Abcam | AB 18258 | Mouse and rat brain lysates. PSD95 knockout mouse and immunoprecipitation. | AB_444362 |
SNAP25 | Abcam | AB 53723 | RAW264.7 cell extracts and blocking peptides. | AB_882623 |
SOD2 | novusbio | NB100–1992 | Rat brain tissue extract. | AB_535862 |
Synaptophysin | Abcam | AB 8049 | Presynaptic vesicles. | AB_2198854 |
TMEM97 | Atlas Antibodies | HPA044795 | Recombinant expression validation using target protein overexpression. | AB_10959306 |