Fig. 3

Strong association between pericytes and Aβ34 in microvessels isolated from frozen human cortex. a Immunostaining of basement membrane marker, Collagen IV (green), Aβ34 (red), pericyte marker, PDGFR-β (blue) and DAPI (blue) in a microvessel isolated from human cortex. (Scale bar 20 μm). b Distribution of PDGFR-β levels (normalized to total protein content) in vessel enriched brain lysates for non-demented controls and AD patients. Graph represent individual values and mean with SD. * (p < 0.05) was determined by a two-tailed unpaired student’s t test. c Correlation of Aβ34 and PDGFR-β levels (normalized to total protein content) in vessel enriched brain lysates in non-demented controls and AD patients. Correlations were calculated using Pearson’s correlation (α = 0.05, CI 95%, two-tailed). d Correlation of Aβ34 and Aβ40 or Aβ42 levels (normalized to total protein content) in vessel enriched brain lysates. For Aβ34 and Aβ40 correlation, Spearman’s correlation (α = 0.05, CI 95%, two-tailed) was used. For Aβ34 and Aβ42 correlation, Pearson’s correlation (α = 0.05, CI 95%, two-tailed) was used. e Distribution of Aβ34/Aβ40 ratio in vessel enriched brain lysates for non-demented controls and AD patients. Graph represent individual values and mean with SD. ** (p < 0.01) was determined by a two-tailed unpaired Mann-Whitney test