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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: The amyloid-β degradation intermediate Aβ34 is pericyte-associated and reduced in brain capillaries of patients with Alzheimer’s disease

Fig. 2

Aβ34 is associated with PDGFR-β + pericytes in small capillaries and Aβ34 immunoreactivity is strongly correlated with pericyte coverage a Merged image of Aβ34 (green) and basement membrane marker, Collagen IV (red) immunostaining. (Scale bar 10 μm) b Merged image of astrocyte marker, GFAP (green), Aβ34 (red) and pericyte marker, PDGFR-β (blue) immunostaining (Scale bar 20 μm). Insert shows magnification of the dashed area. c Merged image of Aβ34 (green), endothelial cell marker, CD31 (red) and pericyte marker, PDGFR-β (blue) immunostaining (Scale bar 20 μm). Insert shows magnification of the dashed area. d Maximum projection of confocal merged image of Aβ34 (green) and pericyte marker PDGFR-β (red). (Scale bar 5 μm). e Correlation of % Aβ34+ vessels with % PDGFR-β + vessels in hippocampus and cortex. % of PDGFR-β + vessels represents the number of PDGFR-β + vessels divided by total number of vessels assessed by Collagen IV immunostaining for each visual field. For hippocampus Pearson’s correlation (α = 0.05, CI 95%, two-tailed), for cortex Spearman’s correlation were (α = 0.05, CI 95%, two-tailed) used. f Braak stage distribution of %PDGFR-β + vessels (pericyte coverage) in hippocampus and cortex. Graphs represent mean with standard deviation (SD). g Diagnosis-based distribution of %PDGFR-β + vessels in hippocampus and cortex. Graphs represent individual values and mean with SD. **** (p < 0.001) was determined by a two-tailed unpaired Mann-Whitney test for hippocampus and student’s t-test for cortex

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