Skip to main content
Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Circadian sleep/wake-associated cells show dipeptide repeat protein aggregates in C9orf72-related ALS and FTLD cases

Fig. 2

Neuropathological investigation of the SCN-related neurons in the hypothalamus of C9orf72 and nonC9orf72 cases. VIP-ir neurons in the hypothalamus at the level of the SON and PVN were referred to as SCN-related neurons (case nonC9–16) (a). The inset shows VIP-immunostaining in neurons and fibers (a). Poly(GA) pathology (visualized by Fast red) was observed in few VIP-ir SCN-related neurons (visualized in brown with DAB) in half of the C9orf72 cases (case C9–3) (b). Inset b1 shows a magnification of a SCN-related neuron with poly(GA) pathology; inset b2 shows a magnification of a VIP-negative neuron with a poly(GA) inclusion. Poly(GA) inclusions (visualized by Fast red) were observed in the area of the SCN-related neurons and fibers (encircled) visualized in brown by DAB (c), however, more poly(GA) inclusions are observed in the area surrounding the SCN-related neurons and fibers (case C9–1) (c). SCN-related neurons were usually spared from pTDP-43 pathology. In some cases, pTDP-43 pathology (black arrows) (visualized by Fast red) was observed in VIP-negative neurons located in between the SCN-related neurons (arrowhead) and fibers (case nonC9–1) (d). The inset in d shows a magnification of a VIP-negative neuron with pTDP-43 pathology. Scale bars represent 1000 μm in a, 100 μm in b, 200 μm in c, 50 μm in d and inset of a, and 5 μm in insets of b and d. VIP-ir stands for vasoactive intestinal peptide-immunoreactive; SCN, suprachiasmatic nucleus; DAB, 3,3′-diaminobenzidine

Back to article page