Fig. 3

HS-68- and pS129 α-syn antibody-positive inclusions in multiple system atrophy (MSA) and Parkinson’s disease (PD). a Fluorescence image of brain tissue section from MSA case 8 (cerebellum) stained with HS-68 and labelled with pS129 α-syn antibody. GCIs (arrowheads) showed complete co-localization between HS-68 and pS129 antibody. b-d Section from PD case 15 (substantia nigra) stained with HS-68 and pS129 α-syn antibody, showing aggregates positive for HS-68 and pS129 (arrowheads) (b); HS-68-positive, pS129-negative, aggregates (arrowhead) (c); weakly HS-68-positive, pS129-positive, aggregates (arrowheads) (d). Channels for single staining are shown in white to enhance visualization. Blue/turquoise structures represent autofluorescent lipofuscin, which is also visible in the channel for HS-68 fluorescence. e Mean HS-68 emission spectra collected from HS-68-positive, pS129-positive (solid line) and HS-68-positive, pS129-negative (dashed line) aggregates indicated by arrowheads in b and c, respectively. A representative experiment is shown. Similar results were obtained using 2 additional cases of each PD and MSA. Scale bars, 20 μm