Table 3 qPCR primers
GENE NAME | FORWARD | REVERSE |
|---|---|---|
Iba-1 | GTCCTTGAAGCGAATGCTGG | CATTCTCAAGATGGCAGATC |
CD-68 | CCTTGTTCTCTTTGATGCAG | GTGATGACAACTAGGATCTT |
CCL-2 | TTTTGTCACCAAGCTCAAGAGA | ATTAAGGCATCACAGTCCGAGT |
CCL-4 | AAACCTAACCCCGAGCAACA | CCATTGGTGCTGAGAACCCT |
GFAP | TCCTGGAACAGCAAAACAAG | CAGCCTCAGGTTGGTTTCAT |
iNOS | AGGAACCTACCAGCTCACTCTG | TTTCCTGTGCTGTGCTACAGTT |
COX-2 | CCACTTCAAGGGAGTCTGGA | AGTCATCTGCTACGGGAGGA |
IL-1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCCAACT |
IL-1α | CAAGATGGCCAAAGTTCGTGAC | GTCTCATGAAGTGAGCCATAGC |
NLRP-3 | AGAAGAGACCACGGCAGAAG | CCTTGGACCAGGTTCAGTGT |
BAD | GAGGAGGAGCTTAGCCCTTT | AGGAACCCTCAAACTCATCG |
BAX | TAGCAAACTGGTGCTCAAGG | TCTTGGATCCAGACAAGCAG |
BIM | CGACAGTCTCAGGAGGAACC | CCTTCTCCATACCAGACGGA |
PUMA | GAGCGGCGGAGACAAGAA | GAGTCCCATGAAGAGATTGTACATGA |
p53 | ACAGTCGGATATGAGCATCG | CCATGGAATTAGGTGACCCT |
LC3 | GATGTCCGACTTATTCGAGAGC | TTGAGCTGTAAGCGCCTTCTA |
BECLIN | AGCTGCCGTTATACTGTTCTG | ACTGCCTCCTGTGTCTTCAATCT |
p62 | GAGGCACCCCGAAACATGG | ACTTATAGCGAGTTCCCACCA |
TIMM10 | GAAGCTCCGTTGCAGTACAG | TCTTGGCAGGACTCGGGAT |
ADAM | AAGGTTGGCTTGACCTGCT | CACCACTCCCAGTTCCTGTT |
SHMT2 | AGTCTATGCCCTATAAGCTCAACCC | GCCGGAAAAGTCGAGCAGT |
PDK4 | GAGGATTACTGACCGCCTCTTTAG | TTCCGGGAATTGTCCATCAC |
POLG | CAGCCTGACCCATAGCCATA | ATTCTCCTTCTGTCAGGTCGAA |
PCG-1α | CTCCA TGCCTGACGGCACCC | GCAGGGACGTCTTTGTGGCT |
NRF2 | CCTCGCTGGAAAAAGAAGTG | GGAGAGGATGCTGCTGAAAG |
TFAM | GCTTCCAGGAGGCTAAGGAT | CCCAATCCCAATGACAACTC |
GAPDH | ATGACATCAAGAAGGTGGTG | CATACCAGGAAATGAGSCTTG |
β-actin | ATGGATGACGATATCGCT | ATGAGGTAGTCTGTCAGGT |