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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: The level of activity of the alternative lengthening of telomeres correlates with patient age in IDH-mutant ATRX-loss-of-expression anaplastic astrocytomas

Fig. 3

Detection of ultra-bright telomeric foci by Telo-FISH in paraffin-embedded sections of tumors, together with detection of DNA by DAPI in the same section, as indicated (40X magnification). The upper chart indicates the mean number of telomeric foci per nucleus, as well as the percentage of nuclei with ultra-bright foci of an intensity over 40 pixels (px), the rate of C-circles and the diagnostic. C-circles values are expressed in arbitrary units (AU; see Materials and methods). The images are representative pictures of tumors; OD: oligodendroglioma; GBM IDHmt: IDH-mutant secondary glioblastoma; AA: anaplastic astrocytoma. Note that the photograph representing the OD tumor (picture a) was taken at a much higher exposure, as indicated, to show that the Telo-FISH signals are indeed present, but are much fainter than in the GBM and AA tumors. For both the OD tumor (a) and AA tumor (b) shown in the upper panels, lower magnifications are provided in the lower panels, as indicated (larger field)

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