Fig. 2

Optimal visualization window for HS-84 with multiphoton microscopy in the rTg4510 mouse brain in vivo. a. Top panel, experimental procedure to characterize HS-84 in the mouse brain in vivo. rTg4510 Tg mice were retro-orbitally injected with HS-84 and longitudinally imaged with multiphoton microscopy for 6 weeks. Bottom panel, pictures show different time points after injection of HS-84 (green). T = 0 represents imaging few minutes after injection. Dextran Texas Red 70,000 Da MW (red) was systematically injected to create a fluorescent angiogram. Scale bar represents 100 μm and applies to all pictures. b. Quantitative analysis of fluorescence intensity of HS-84 binding to NFTs. Normalized to t = 24 h. Optimal imaging time was determined to be 1 week after HS-84 injection in the rTg4510 Tg mouse. n = 111 cells from 2 mice. c. Quantitative analysis of fluorescence intensity HS-84 in the blood circulation. Normalized to t = 24 h. n = 48 vessels from 2 mice. d. Dynamics of NFTs formation in the rTg4510 Tg mouse. Top panel, experimental procedure to characterize formation of NFTs in the mouse brain in vivo. rTg4510 Tg mouse with a window already implanted was intravenously (retro-orbital) injected with HS-84 and longitudinally imaged with multiphoton microscopy for 3 consecutive days. Bottom, pictures show 3 successive days 1 week after injection of HS-84 (green). Dextran Texas Red 70,000 Da MW (red) was systematically injected. Scale bar represents 50 μm and applies to all pictures. New NFTs can be observed within 1 day