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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: N368-Tau fragments generated by legumain are detected only in trace amount in the insoluble Tau aggregates isolated from AD brain

Fig. 6

Insoluble levels of N368-cleaved and uncleaved Tau are higher in AD than control hippocampus. Insoluble Tau extracts P3 and PS1 from human hippocampus and cerebellum were prepared as described in the Methods. Levels of N368-cleaved ad uncleaved Tau were measured by LC-MS/MS. a-b. In the sarkosyl-insoluble P3 extracts, levels of both N368-cleaved (a) and uncleaved Tau (b) are significantly higher in AD hippocampus compared to control hippocampus (p < 0.0001) and AD cerebellum (a, p < 0.0001; b, p = 0.0003). c. In the sarkosyl-insoluble P3 extracts, the percentage N368-cleaved Tau over uncleaved Tau is significantly lower in AD hippocampus than control hippocampus (p = 0.001) and AD cerebellum (p = 0.001). d-e. In the sarkosyl-insoluble PS1 extracts, levels of both N368-cleaved (d) and uncleaved Tau (e) are significantly higher in AD hippocampus compared to control hippocampus (p < 0.0001) and AD cerebellum (d, p = 0.002; e, p = 0.001). f. In the sarkosyl-insoluble PS1 extracts, the percentage N368-cleaved Tau over uncleaved Tau is significantly lower in AD than control hippocampus (p < 0.0001) and AD cerebellum (p = 0.009). AD hippocampus, n = 10; AD cerebellum, n = 6; Control hippocampus, n = 10. Hp, hippocampus; Cb, cerebellum. In all graphs, data points, mean and standard deviation are shown. **p < 0.01

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