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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: N368-Tau fragments generated by legumain are detected only in trace amount in the insoluble Tau aggregates isolated from AD brain

Fig. 5

Soluble levels of N368-cleaved Tau are similar in AD and control hippocampus. Soluble Tau extracts SS1 and S3 from human hippocampus and cerebellum were prepared as described in the Methods. Levels of N368-cleaved ad uncleaved Tau were measured by LC-MS/MS. a. In the sarkosyl-soluble SS1 extracts, levels of N368-cleaved-Tau are comparable in AD and control hippocampus (p = 0.654) and AD hippocampus vs cerebellum (p = 0.310). b. Levels of uncleaved-Tau are significantly lower in the sarkosyl-soluble SS1 extracts of human AD than control hippocampus (p < 0.0001). Uncleaved Tau is similar in AD hippocampus and cerebellum (p = 0.380). c. In the sarkosyl-soluble SS1 extracts, the percentage N368-cleaved Tau over uncleaved Tau is significantly higher in AD than control hippocampus (p = 0.0001) and is similar in AD hippocampus and cerebellum (p = 0.304). d. In the sarkosyl-soluble S3 extracts, levels of N368-cleaved Tau are comparable in AD hippocampus vs. control hippocampus (p = 0.970) or AD hippocampus vs. cerebellum (p = 0.296). e. In the sarkosyl-soluble S3 extracts, levels of uncleaved Tau are comparable in AD vs. control hippocampus (p = 0.454). In AD, uncleaved Tau is significantly lower in the cerebellum than hippocampus (p = 0.030). f. In the sarkosyl-soluble S3 extracts, the percentage N368-cleaved Tau over uncleaved Tau is comparable in AD vs control hippocampus (p = 0.505). In AD, N368-cleaved Tau percentage is significantly higher in cerebellum vs hippocampus (p = 0.033). AD hippocampus, n = 11; AD cerebellum, n = 6; Control hippocampus, n = 10. Hp, hippocampus; Cb, cerebellum. In all graphs, data points, mean and standard deviation are shown. *p < 0.05; **p < 0.01

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