Fig. 4

Total levels of N368-cleaved Tau do not change in the total homogenate brain of Tau transgenic mice. a. Insoluble Tau inclusions in the brainstem and cortex of Thy1.P301STau transgenic mice at 2 and 5 months of age. Tau inclusions are immunostained using the phosphorylation- and conformation-dependent AT100 antibody. Scale bars: 100 μm. b, c. In total homogenate of Thy1.P301STau transgenic cortex (b) and brainstem (c), N368-cleaved Tau levels are similar at 2.5 and 5.8 months of age (cortex, p = 0.675; brainstem, p = 0.850; n = 10/group). The quantification of Tau in the total mouse brain homogenate was performed by LC-MS/MS as depicted in Fig. 3a. In all graphs, data points, mean and standard deviation are shown