Fig. 5
From: Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo
Depletion of BICD2 in the cortex impairs neuronal maturation and survival. a. Coronal cryo-sections of E17.5 cortices from cell-type-specific conditional Bicd2 KO mice and their control littermates - Bicd2fl/fl;Nex-Cre+/− (=Nex-KO), Bicd2fl/fl;Emx1-Cre+/− (=Emx1-KO), Bicd2fl/fl;Nex-Cre−/− (=Nex-WT) and Bicd2fl/fl;Emx1-Cre−/− (=Emx1-WT) respectively - were stained against cleaved Caspase-3 (Cas3, red) as marker for apoptotic cell death and NeuN (green) as marker for mature neurons. Scale bars are 100 μm. b. Zoom of selected areas indicated in (a) show the Cas3 (top panels) and NeuN (lower panels) immunostaining in the CP. c + d. Graphical representation of the relative position of Cas3+ cells over the cortical longitude from ventricular (VS) to pial surface (PS) and 156.3 μm in width (both in %) in Nex-WT and Nex-KO (c) and Emx1-WT and Emx1-KO (d) cortices. e. Distribution and accumulated number of Cas3+ cells from 3 experiments as relative position over the cortical longitude from VS to PS (in %). Red circles are individual Cas3+ cell locations of representative samples (N = 5–9, n = 7–282). f. Number (10− 3) of Cas3+ cells per μm2 (N = 5–9, n = 7–282). g + h. Graphical representation of the relative position of NeuN+ cells over the cortical longitude from ventricular (VS) to pial surface (PS) and 156.3 μm in width (both in %) in Nex-WT and Nex-KO (c) and Emx1-WT and Emx1-KO (d) cortices. i. Distribution and accumulated number of NeuN+ cells from 3 experiments as relative position over the cortical longitude from VS to PS (in %). Red circles are individual NeuN+ cell locations of representative samples (N = 3, n = 28–300). j. Number of NeuN+ cells per μm2 (N = 3, n = 28–300). CP: cortical plate, IZ: intermediate zone, PS: pial surface, SP: subplate, SVZ: subventricular zone, VS: ventricular surface. *** p < 0.001, ** p < 0.005, ns = not significant; error bars are ±SEM. used tests: Kruskal Wallis test with Dunn’s multiple comparisons (e, i), One Way ANOVA with Tukey’s multiple comparisons (f, j)