Fig. 1
From: Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo
Neuronal migration and lamination in the cortex depends on the neuron-specific expression and function of BICD2 in excitatory radial migrating neurons. a. Coronal cryo-sections of E17.5 cortices from cell-type-specific conditional Bicd2 KO mice and their control littermates - Bicd2fl/fl;Nex-Cre+/− (=Nex-KO), Bicd2fl/fl;Emx1-Cre+/− (=Emx1-KO), Bicd2fl/fl;Nex-Cre−/− (=Nex-WT) and Bicd2fl/fl;Emx1-Cre−/− (=Emx1-WT) respectively - were stained against the upper-layer (II/III) marker SATB2 (red) and the intermediate basal progenitor marker TBR2 (green). DAPI is shown in blue. Scale bars are 100 μm. b + c. Graphical representation of the relative position of SATB2+ cells over the cortical longitude from ventricular (VS) to pial surface (PS) and 187.5 μm in width (both in %) (left panels); and quantification of the relative frequency of SATB2+ cells over the cortical longitude (%, binned in centers) and their gaussian distribution (right panels) for Nex-WT and Nex-KO mice (b) and Emx1-WT and Emx1-KO mice (c). d + e. Graphical representation of the relative position of TBR2+ over the cortical longitude from VS to PS and 187.5 μm in width (both in %) (left panels); and quantification of the relative frequency of TBR2+ cells over the cortical longitude (%, binned in centers) and their gaussian distribution (right panels) for Nex-WT and Nex-KO mice (d) and Emx1-WT and Emx1-KO mice (e). f. Relative amount of SATB2+ cells in VZ, SVZ/IZ and CP. For SATB2+ cells, we have counted cell location for at least 3 mice (N = 3–6, average cell position per mice is represented as individual data points in the graph) for each genotype coming from at least 2 different litters. Between 237 and 640 cells have been counted per mouse (n = 237–640). g. Relative amount of SATB2+ cells in the cortex, based on ratio of SATB2+/DAPI+ cells. h. Number (10− 3) of TBR2+ cells per μm2 (N = 5–9, n = 150–464). CP: cortical plate, IZ: intermediate zone, PS: pial surface, SVZ: subventricular zone, VS: ventricular surface, VZ: ventricular zone. *** p < 0.001, ** p < 0.005, * p < 0.05, ns = not significant; error bars are ±SEM. Used tests: One Way ANOVA with Sidak’s multiple comparison (f), Mann-Whitney U test (g, h)