Fig. 5

Immunohistochemical staining of EGFR, MET, CA12, AR, and PSMA. a/b EGFR staining of tumour N15–12 (negative in the smMIP assay) was negative in the frozen section, but partly positive in the FFPE section, indicating intratumoural heterogeneity of EGFR expression. High EGFR gene expression in tumour N16–13 was consistent with protein expression. IHC staining for MET (c), CA12 (d), and AR (e) of tumour samples with low (upper panels) and high (lower panels) expression levels of MET, CA12 and AR respectively in the t/RNA-NGS assay. Expression levels in FPM are stated with the sample name. f Staining for PSMA showed endothelial staining in gliomas with high PSMA FPM values, as indicated with the yellow arrowheads in the lower panel. In gliomas with low expression (N14–05), no staining was observed (red arrowheads in upper panel point at blood vessels). Original magnification for upper panel in A, 5x. For lower panel of A and other panels, magnification is 10x. Yellow annotated bar for 5 × 500 μm, for 10 × 200 μm