Fig. 4

Immunohistochemistry for pTDP-43 (a, d, g and j), nTDP-43 (b, e, h and k) and p62 (c, f, i and l) in serial sections of myofibers in the diaphragm from patients with ALS. a-c Normal-looking myofibers containing fine granular structures immunopositive for nTDP-43 (b) and p62 (c), but not for pTDP-43 (a) (case A-6). d-f Short linear inclusions are immunopositive for pTDP-43 (1 in d), nTDP-43 (e) and p62 (f) (case B-16). g-i Accumulated short linear inclusions (arrows; 2 in g) and small dense filamentous inclusions (arrowheads, 3 in g) are immunopositive for pTDP-43 (g), nTDP-43 (h) and p62 (i) (case B-16). j-l Larger dense filamentous inclusions (4 in j) are immunopositive for pTDP-43 (j), nTDP-43 (k) and p62 (l) (case A-6). Bars = 20 μm