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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: A post-translational modification signature defines changes in soluble tau correlating with oligomerization in early stage Alzheimer’s disease brain

Fig. 3

Oligomerization of tau can be monitored with Tau12-Tau12 or T22-Tau12 ELISA. a) Fluorescence intensities of ThT assays showing aggregation of recombinant full-length tau over time. Seeds alone (tau aa256–368), buffer alone and full-length tau alone were used as controls. The signal for tau with seeds increases exponentially until app. 8 h of incubation (n = 3). Analysis of aggregates by b) Tau12-Tau12 ELISA assay and c) T22-Tau12 ELISA assay. Both methods yield a higher signal for tau with seeds after 48 h of incubation, which is abolished after boiling in SDS-containing buffer (n = 3). d) Dot blot analysis of native samples with T22 antibody: seeds alone, tau alone and tau with seeds at 0 h and 48 h. Two-way Anova for b and c: **, p < 0.01, ***, p < 0.001, ****, p < 0.0001

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