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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Synergistic toxicity in an in vivo model of neurodegeneration through the co-expression of human TDP-43M337V and tauT175D protein

Fig. 6

Microgliosis is increased in the hippocampus of ChAT-tTA/TRE-TDP-43M337V rats co-expressing pathogenic human tauT175D protein. a Representative photomicrographs showing IBA1 stained microglia in ChAT-tTA control and tauT175D expressing ChAT-tTA/TRE-TDP-43M337V rat hippocampus. Insets show an activated microglial cell with enlarged cell body in ChAT-tTA/TRE-TDP-43M337V transgenic rat brain and resting microglial cell in ChAT-tTA control. Light microscopy low magnification images were taken with 20x objective, insets taken with 100x oil immersion objective. Scale bar = 50 μm. b Quantification of IBA1 staining across the field of view shows increased coverage (proxy for microglial activation) in ChAT-tTA/TRE-TDP-43M337V rats with human tauT175D expressed in the hippocampus. All quantification represents either the ChAT-tTA control group (non-expressing for TDP-43M337V or any GFP construct) or hippocampal GFP-tau expressing groups on ChAT-tTA/TRE-TDP-43M337V transgenic background (GFP = green fluorescent protein, tauWT = GFP-tagged tauWT, tauT175D = GFP-tagged tauT175D human tau). *p < 0.05 between indicated groups. c Fluorescence microscopy of the hippocampus of wild type rats (no ChAT/no TDP-43 transgenes; top two rows) and rats expressing human TDP-43 (bottom two rows) with tauWT (top row and third row) or tauT175D (second and four rows) co-probed with antibodies to GFP (fusion protein with tau) and IBA1. White arrows indicate IBA1 positive microglial cells in close-proximity to GFP-positive (tau positive) hippocampal neurons. Three animals were examined for each group and photomicrographs shown are representative for each group

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