Fig. 5

Hippocampal expression of human GFP-tagged tau protein using AT8 antibody against paired helical filament tau in parental lines and rats expressing TDP-43 with GFP and/or tau expression. Representative sections from the CA1 region of the hippocampus in rats. Parental lines (ChAT-tTA and TRE-TDP-43^M337V) were not injected with any rAAV9 (top two rows). Rats expressing TDP-43 (ChAT-tTA/TRE-TDP-43^M337V were injected with either rAAV9 encoding GFP alone (GFP), or GFP-fused wildtype tau (tau^WT) or GFP-fused tau^T175D (tau^T175D). Note the staining in the ChAT-tTA/TRE-TDP-43^M337V rats expressing tau^T175D (brown, bottom row; n = 3 for each group). Photos in the left column were taken with 40X objective, right column with oil immersion 100X objective. Scale bar = 20 μm