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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Synergistic toxicity in an in vivo model of neurodegeneration through the co-expression of human TDP-43M337V and tauT175D protein

Fig. 2

Cerebral expression of human GFP-tagged tau protein constructs and human TDP-43M337V. a Composite image of hippocampus from ChAT-tTA/TRE-TDP-43 rat expressing tauT175D probed for GFP shows expression of GFP-tagged tauT175D protein throughout the hippocampus with prominent neuronal and neuritic expression in the CA2 region (brown). This same expression pattern was observed for all three AAV9 injected groups (GFP, GFP-tagged tauWT, and GFP-tagged tauT175D; n = 3 for each group). b TRE-TDP-43M337V hippocampus probed for human TDP-43 reveals no expression of human TDP-43 confirming that TDP-43 is not expressed without the ChAT-tTA transgene (n = 3). c and d ChAT-tTA/TRE-TDP-43M337V transgenic rat hippocampus probed for human TDP-43 showed a variety of expression during DOX withdrawal, ranging from extremely low levels (c) to robust TDP-43 expression (d). e TRE-TDP-43M337V rat cortex probed for human TDP-43 reveals no evidence of expression (left) while ChAT-tTA/TRE-TDP-43M337V rat cortex shows human TDP-43 expression that is predominantly nuclear (right). All composite images taken with a 20x objective. Scale bar = 10 μm

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