Table 4 Morphologic criteria
Number of affected fibers | |||
|---|---|---|---|
HE | Internalized nuclei | + | < 10% |
++ | 10–30% | ||
+++ | > 30% | ||
Fibrotic areas | + | < 5% | |
++ | 5–30% | ||
+++ | > 30% | ||
Vacuoles | + | < 5% | |
++ | 5–30% | ||
+++ | > 30% | ||
Ring Fibers | + | < 5% | |
++ | 5–10% | ||
+++ | > 10% | ||
Necrosis | + | < 2% | |
++ | 2–5% | ||
+++ | > 5% | ||
Regeneration | + | < 2% | |
++ | 2–5% | ||
+++ | > 5% | ||
PAS | Augmented intensity | + | < 5% |
++ | 5–30% | ||
+++ | > 30% | ||
Positive vacuoles | + | < 5% | |
++ | 5–30% | ||
+++ | > 30% | ||
IHC | SQSTM1/p62 | + | < 5% |
++ | 5–30% | ||
+++ | > 30% | ||
EM | Intermyofibrillar glycogen accumulation | + | < 5% |
++ | 5–30% | ||
+++ | > 30% | ||
Autophagosomes | + | < 5% | |
++ | 5–30% | ||
+++ | > 30% | ||
Autophagic structures | + | < 5% | |
++ | 5–30% | ||
+++ | > 30% |