Fig. 5

Rab39b and GluR1 levels are reciprocally altered in the post-synaptic compartment of C9-KO mice. a Western blot analysis of pre-synaptic density fractions (P4) from C9-WT and C9-KO mice (n = 2 mice per sample; n = 4 biological replicates per group). Post-Synaptic Density Protein 95 (PSD95); TAR DNA Binding Protein 43 (TDP-43); Chromosome 9 open reading frame 72 (C9orf72); Smith-Magenis syndrome Chromosomal Region candidate gene 8 (Smcr8); Ras-related proteins Rab39b; Glutamate receptor 1 (GluR1); glutamate receptor 2 (GluR2); N-methyl-D-aspartic acid receptor 1 (NMDAR1). b Bar plots of mean immunoblot band density for each antibody probed in the P4 fraction. x-axis = C9-WT versus C9-KO for each antibody; y-axis = relative density (ratio relative to wild-type); error bars = standard deviation; *Q<0.01. Representative immunofluorescent micrographs of c Rab39b and d GluR1 in the dorsal hippocampus of C9-WT versus C9-WT mice. Scale = 200 μm. Quantification of e Rab39b and f GluR1 fluorescence intensity in the dorsal hippocampus of C9-WT versus C9-WT mice (n = 4 animals; n = 3 micrographs per animal); y-axis = relative fluorescence intensity as a percentage (%) of C9-WT #p<0.001 for Rab39b; #p<0.05 for GluR1