Fig. 3

Rab3a protein levels are increased in the synaptosomal fraction of C9-KO mice relative to C9-WT mice. a Immunoblots from the post-nuclear input fraction (S1) from C9-WT and C9-KO mice (n = 2 mice per sample; n = 4 biological replicates per group). Loading confirmed by glyceraldehyde 3-phosphate dehydrogenase (GAPDH); Chromosome 9 open reading frame 72 (C9orf72); Smith-Magenis syndrome Chromosomal Region candidate gene 8 (Smcr8); Ras-related protein Rab39b; Glutamate receptor 1 (GluR1). b Immunoblots from the synaptosomal (SYN) fraction. c Bar plots of mean immunoblot band density for each antibody probed in the SYN fraction. x-axis = C9-WT versus C9-KO for each antibody; y-axis = relative density (ratio relative to wild-type); error bars = standard deviation; *Q<0.01. Synaptophysin (Syp); Post-Synaptic Density Protein 95 (PSD95); TAR DNA Binding Protein 43 (TDP-43); Rab family members Rab3a, Rab5, Rab11, Rab39b; Glutamate receptor 1 (GluR1); glutamate receptor 2 (GluR2); N-methyl-D-aspartic acid receptor 1 (NMDAR1)