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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels

Fig. 2

C9orf72 and Rab39b are localized to post-synaptic densities. a Immunoblot analysis of affinity purified PSD-95 complexes from C9-WT mice. PSD = PSD-95 antibody; UB = unbound fraction; IgG = rabbit IgG; wash = last wash. Synaptophysin (Syp); Post-Synaptic Density Protein 95 (PSD95); TAR DNA Binding Protein 43 (TDP-43); Chromosome 9 open reading frame 72 (C9orf72); Smith-Magenis syndrome Chromosomal Region candidate gene 8 (Smcr8); Ras-related proteins Rab3a, Rab5, Rab11, Rab39b; Glutamate receptor 1 (GluR1); glutamate receptor 2 (GluR2); N-methyl-D-aspartic acid receptor 1 (NMDAR1). Glial fibrillary acid protein (Gfap); Neurofilament Light Chain (Nefl). b Low magnification immunofluorescent micrographs of C9orf72, PSD-95, and DAPI or C9orf72, Synaptoporin (Synpr), and DAPI in the dorsal hippocampus of C9-WT and C9-KO mice. Scale = 200 μm. c High magnification immunofluorescent micrographs of C9orf72 with either PSD-95, microtubule associated protein 2 (MAP2), Synpr, or Rab39b in the CA3 region of the hippocampus from C9-WT and C9-KO mice. Insets contain arrowheads which indicate co-detection of fluorescent signal between the red and green channels. Scale = 20 μm

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