Fig. 1
From: Synaptic localization of C9orf72 regulates post-synaptic glutamate receptor 1 levels
Biochemical distribution of C9orf72 and Rab proteins in the pre- and post-synaptic compartments of wild-type murine forebrains. a Flowchart for the fractionation of synaptosomes, pre-synapses, and post-synaptic densities (PSDs) used in the current study. S1 = post-nuclear supernatant; P1 = nucleus and debris; S2 = crude cytoplasm; P2 = crude synaptosomes; SYN = pure synaptosomes; S3 = presynaptic terminals; P3 = crude PSDs; S4 = Triton X-100-KCl treated P3 supernatant; P4 = semi-pure PSDs; S4* = Triton X-100-KCl treated crude PSD supernatant; P4* = PSDs; PSD-IP = pure PSDs. b Distribution of markers across synaptic fractions in C57BL/6 mice. Synaptophysin (Syp); Post-Synaptic Density Protein 95 (PSD-95); TAR DNA Binding Protein 43 (TDP-43); Chromosome 9 open reading frame 72 (C9orf72); Smith-Magenis syndrome Chromosomal Region candidate gene 8 (Smcr8); Ras-related proteins Rab3a, Rab5, Rab11, Rab39b; Glutamate receptor 1 (GluR1); glutamate receptor 2 (GluR2); N-methyl-D-aspartic acid receptor 1 (NMDAR1)