Fig. 5

CD4⁺ but not CD8⁺ cells are differentially present in GBM subtypes. a and c show representative classical, mesenchymal, neural and proneural GBMs stained for CD4 and CD8 respectively. In b, mesenchymal GBM have a higher percentage of CD4 positive cells compared to the other GBM subtypes (of note, CD4 may be expressed in cells other than T lymphocytes, such as monocytes and macrophages). No differences were found in CD8 infiltrate (d). Scale bar, 100 μm. Arrowheads indicate representative positive cells. CL, classical; MES, mesenchymal; N, neural; PN, proneural. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001