Skip to main content
Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: The p75NTR neurotrophin receptor is required to organize the mature neuromuscular synapse by regulating synaptic vesicle availability

Fig. 7

Unaltered BDNF/TrkB signaling at the NMJ of p75NTR−/− mice. a TA muscle cryosections from WT and p75NTR−/− mice were double-labeled with antibodies (green) to detect p-TrkB Y816 or BDNF, along with BTX (red). bc Quantification of the percentage of NMJs labeled with BNDF (b) or with p-TrkB Y816 (c). d Total protein samples of TA muscles from WT and p75NTR−/− (n = 3) mice were analyzed by Western blot using specific antibodies to detect p-TrkB Y816, total TrkB, and BDNF. The levels of GAPDH were used as loading control. ef BDNF/TrkB signaling was estimated by band intensity densitometry of BDNF (e) and by the Y816 phosphorylation of both, the glycosylated (145 kDa) and the unprocessed (110 kDa) forms of TrkB and expressed as a ratio of the corresponding bands detected with an anti TrkB antibody (f). n.s., non-significant, unpaired t-test

Back to article page