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Table 6 Strategies for long-term storage of perfusion-fixed brain tissue

From: Perfusion fixation in brain banking: a systematic review

Study Overall method Study type Tissue Preservative agent(s) Temperature Storage duration
Alvernia 2010 [3] Immersion in fixative Surgical training Separated head 10% Formalin and 10% ethyl alcohol 4 °C Up to 4 years
Benet 2014 [9] Immersion in fixative Surgical training Separated head 10% formaldehyde or 10% custom solution (ethanol 62.4%, glycerol 17%, phenol 10.2%, formaldehyde 2.3%, and water 8.1%) 5 °C Up to a year
Insausti 1995 [42] Cryoprotection and freezing Histology 1 cm-thick coronal tissue blocks Solutions of 10 and 20% glycerol in 0.1 M phosphate buffer and 2% dimethylsulfoxide −80 °C NR
Lyck 2008 [58] Immersion in fixative Histology Whole brain 0.1% paraformaldehyde in 0.15 M Sørensens phosphate buffer (pH 7.4) 4 °C Up to 4 years
Sutoo 1994 [85] Cryoprotection and freezing Histology 1 cm-thick coronal tissue blocks Buffered 5% sucrose −80 °C NR
Waldgovel 2006 [96] Cryoprotection and freezing Histology Tissue blocks (many 1 cm-thick) 20–30% sucrose in 0.1 M phosphate buffer with 0.1% sodium azide −80 °C NR
Welikovitch 2018 [99] Cryoprotection and freezing Histology Brain sections 1.1 M sucrose, 37.5% ethylene glycol in PBS −20 °C NR
  1. If a study did not report the use of a long-term storage method, then it is not included in this table. NR: Not reported, PBS: Phosphate-buffered saline