Table 6 Strategies for long-term storage of perfusion-fixed brain tissue

Alvernia 2010 [3]

Immersion in fixative

Surgical training

Separated head

10% Formalin and 10% ethyl alcohol

4 °C

Up to 4 years

Benet 2014 [9]

Immersion in fixative

Surgical training

Separated head

10% formaldehyde or 10% custom solution (ethanol 62.4%, glycerol 17%, phenol 10.2%, formaldehyde 2.3%, and water 8.1%)

5 °C

Up to a year

Insausti 1995 [42]

Cryoprotection and freezing

Histology

1 cm-thick coronal tissue blocks

Solutions of 10 and 20% glycerol in 0.1 M phosphate buffer and 2% dimethylsulfoxide

−80 °C

NR

Lyck 2008 [58]

Immersion in fixative

Histology

Whole brain

0.1% paraformaldehyde in 0.15 M Sørensens phosphate buffer (pH 7.4)

4 °C

Up to 4 years

Sutoo 1994 [85]

Cryoprotection and freezing

Histology

1 cm-thick coronal tissue blocks

Buffered 5% sucrose

−80 °C

NR

Waldgovel 2006 [96]

Cryoprotection and freezing

Histology

Tissue blocks (many 1 cm-thick)

20–30% sucrose in 0.1 M phosphate buffer with 0.1% sodium azide

−80 °C

NR

Welikovitch 2018 [99]

Cryoprotection and freezing

Histology

Brain sections

1.1 M sucrose, 37.5% ethylene glycol in PBS

−20 °C

NR