Fig. 6
STAT6 regulates glioma cell viability. a, b U87MG cells transfected with CONsi or STAT6si were exposed to 0% O2 for 5 days, and then assessed by WST viability assay (a) and LIVE (green)/DEAD (red) assay (b). Results are presented as means ± SD (error bars) of three independent experiments (*p < 0.05 vs. CONsi-transfected cells). c Immunoblot of STAT6, cleaved (cl) caspase 3 (Cas 3), and cl-Cas 7 in siRNA-transfected U87MG cells exposed to 0% O2 for indicated days. d Immunoblot of STAT6, HIF-1α, and cl-Cas 7 in indicated siRNA-transfected U87MG cells exposed to 0% O2 for indicated times. e, f Two days after transfecting with CONsi or STAT6si, U373MG cells were incubated with or without 5-Aza (300 nM) for 2 days, and incubated in fresh medium (without drugs) for 6 days. Flow cytometry dot plots of annexin V and propidium iodide (PI) staining and summary data showing the percentage of annexin V-positive cells (e) and immunoblot of STAT6, cl-Cas-3 and -7, and cl- PARP (f). Results are presented as means ± SD (error bars) of three independent experiments (*p < 0.05). g Flow cytometry histogram plots of PI-stained DNA content (left) and summary data (right) showing the percentage of cells in each cell cycle phase. Results are presented as means ± SD (error bars) of three independent experiments (*p < 0.05)