Fig. 4
STAT6 negatively regulates mTOR signaling. a Schematic of downstream mTOR signaling. b Immunoblot of HIF-1α, p-S6K, p-S6 and STAT6 in CONsi or STAT6si -transfected U87MG cells exposed to 0% O2 for 18 h, with or without rapamycin (1 nM). c Immunoblot of the indicated proteins (top) and summary data (bottom) showing actin-normalized p-S6K and p-S6 levels expressed as fold-changes relative to that in CONsi-transfected cells exposed to 21% O2 in siRNA-transfected U87MG cells exposed to 0% O2 for 18 h. Results are presented as means ± SD (error bars) of three independent experiments (*p < 0.05). d Immunoblot of the indicated proteins (left) and summary data (right) showing actin-normalized p-4E-BP1 and 4E-BP1 levels expressed as fold-changes relative to that in CONsi-transfected cells exposed to 21% O2 in siRNA-transfected U87MG cells exposed to 0% O2 for 18 h. Results are presented as means ± SD (error bars) of three independent experiments (*p < 0.05). e RT-qPCR of 4E-BP1 in siRNA-transfected U87MG cells exposed to 0% O2 for 18 h. Results are presented as means ± SD (error bars) of three independent experiments (*p < 0.05). f Immunoblot of eIF4G in eIF4E IPs from U87MG cells transfected with CONsi or STAT6si and exposed to 0% O2 for 10 h