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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Epigenetic downregulation of STAT6 increases HIF-1α expression via mTOR/S6K/S6, leading to enhanced hypoxic viability of glioma cells

Fig. 1

STAT6 is downregulated in glioma. a Immunoblot of the indicated STATs in non-tumor brain tissue (NTT), low-grade glioma tissue (LGG), and high-grade glioma tissue (HGG) (top) and summary data (bottom) showing actin-normalized levels of STATs. b Representative immunohistochemical staining of STAT6 in normal and GBM tissue (top) and summary data (bottom) showing the quantification (H-score, see Materials and Methods) of STAT6 staining. c Immunoblot of the indicated STATs in paired normal (NTT5), primary (HGG9–1) and recurrent GBM (HGG9) samples. N, normal; T, tumor. d Immunoblot of STAT6 in GBM (HGG17 and HGG18) and anaplastic oligodendroglioma (HGG14) and paired normal tissues from each. NTT4, HGG17-paired; NTT6, HGG18-paired; NTT7, HGG14-paired. e STAT6 mRNA levels in NTT (n = 3), LGG (n = 3) and HGG (n = 3), determined by RT-qPCR. Transcript levels were normalized to GAPDH or actin levels. f Expression of STAT6 from GEO data set GDS1962 containing 23 non-tumor (NTT), 45 grade II (LGG), and 31 and 81 grade III and IV (HGG) samples (*p < 0.05, **p < 0.005, and ***p < 0.0005 vs. NTT). g Expression of STAT6 from Oncomine data set TCGA brain consisting of normal brain (n = 10) and brain glioblastoma (n = 542) samples

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