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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Composition of the Intranuclear Inclusions of Fragile X-associated Tremor/Ataxia Syndrome

Fig. 2

FXTAS inclusions are largely made up of protein and contain a smaller component of RNA, but do not contain significant amounts of DNA. DNase treatment did not affect the quantity, integrity, or level of autofluorescence exhibited by isolated inclusions, detected at 488nm, 555nm; not visualized at 647nm without several fold increase in brightness. RNase treatment did not affect the quantity of inclusions seen, but the autofluorescence levels exhibited by inclusions was decreased. Postprocessing to increase the brightness of RNase treated inclusions revealed that the integrity of the inclusions appeared compromised. Protease treatment completely abolished visualization of inclusions using autofluorescence, and no inclusions were observed. Unless specified (bright), all images were acquired for 208.1 ms

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