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Table 3 Overview of the different cryo-substitution cocktails

From: Optimization of ultrastructural preservation of human brain for transmission electron microscopy after long post-mortem intervals

 

Cryo-substitution media

Cocktail I

2% osmium tetroxide and 0.2% uranyl acetate in water free acetone

Cocktail II

0.2% uranyl acetate in water free acetone

Cocktail III

water free acetone