Fig. 10

Immunoblotting comparison of αsyn species in LBD cingulate cortex. High salt (HS) and SDS/urea fractions were obtained from the cingulate cortex of 2 control cases (lanes 1–2) and 3 LBD cases (lanes 3–5). 20 μg of lysate for each case and fraction were subject to western blot analysis using a panel of 4 antibodies, as indicated. In the HS fraction, all antibodies demonstrate similar amounts of monomeric FL αsyn except for one of the LBD cases which had a diminished level. In the SDS/Urea fractions, monomeric FL αsyn is present in high amounts for all LBD cases while the 2 controls have almost no αsyn in this fraction. Although higher molecular mass M1 and M2 bands are present in the cingulate SDS/urea fraction, truncation bands T1 and T2 are not as apparent which represents a biochemical difference between αsyn in the amygdala and MTL compared with cingulate cortex. The relative mobilities of molecular mass protein markers are identified on the left of the blots