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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Characterization of the human myelin oligodendrocyte glycoprotein antibody response in demyelination

Fig. 2

Human MOG Ab binding is influenced by conformational changes to native MOG. a and b High surface expression of native-MOG was comparable to formaldehyde-fixed MOG by immunocytochemistry (a, scale bar = 20 μm) and flow cytometry (b). c 78/139 (56%) native-MOG Ab+ children and 79/148 (53%) adult sera were positive for fixed-MOG Ab, whereas fixed-MOG Ab were not detected in all paediatric (n = 24) and adult (n = 24) control sera, and all native-MOG Ab- paediatric (n = 24) and adult (n = 24) sera (grey). Dotted line represents the positivity threshold (mean of controls +3SD). Fixed-MOG Ab positivity is shown between brackets. d In the fixed flow assay, 59% of children (36/61) and adults (41/69) who failed to bind fixed-MOG (filled-red) had low native-MOG Ab titers. A conformational insensitivity was observed in 10/46 children (22%) and 8/49 adults (16%) who could bind fixed-MOG despite low native-MOG Ab titers (empty-blue in low category). However, a sensitivity to conformation was seen in 25/61 (41%) children and 28/69 (41%) adults had mid to high native-MOG Ab titers. Number and percentage of patients negative in the fixed flow assay are shown in brackets. e Fixed-MOG Ab titers were higher in BON than UON patients (P = 0.01). f Adults seronegative by fixed flow assay predominantly presented with monophasic and relapsing UON (52%, 25/48), and BON (27%, 13/48), and 51% of non-binders had a relapsing course. Relapsing ADEM is multiphasic ADEM according to [30]. Unknown or other phenotypes (n = 16) and phenotypes with less than two patients are not represented (n = 5). g In the fixed biochip assay, reduced assay sensitivity was observed in 11/19 children (58%) and 13/24 adults (54%) who could not bind fixed-MOG in the fixed biochip assay (filled- red) and had low titers of native-MOG Ab. Conformational sensitivity was observed in 8/19 (42%) children and 11/24 adults (46%) who were negative by fixed biochip assay despite mid to high native-MOG Ab titers, and conformational insensitivity in 13/24 children (34%) and 7/20 (35%) adults positive in the fixed biochip assay but with low native-MOG Ab (empty-blue in low category). h Most adults seronegative by fixed biochip assay presented with monophasic and relapsing UON (57%), followed by BON (28%), and 64% had a relapsing course. Unknown phenotypes (n = 5) and phenotypes with less than two patients are not represented (n = 5). Relapsing ADEM is multiphasic ADEM according to [30]. θMRW = mean residue weight ellipticity, Ab = antibody, native-MOG1–117 = extracellular MOG, fixed-MOG = fixed MOG, fixed-CTL = fixed transduced control cells, MFI = median fluorescence intensity, UON = unilateral optic neuritis, BON = bilateral optic neuritis, ON mixed = combination of BON and UON, ON/TM = simultaneous ON and TM, LETM = longitudinally extensive transverse myelitis, relapsing ADEM* = multiphasic ADEM

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